The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA.
Identifieur interne : 003D02 ( Main/Exploration ); précédent : 003D01; suivant : 003D03The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA.
Auteurs : I G Barr [Australie] ; M. Mccaig ; C. Durrant ; R. ShawSource :
- Vaccine [ 0264-410X ] ; 2006.
Descripteurs français
- KwdFr :
- Grippe humaine (virologie), Grippe humaine (épidémiologie), Humains (MeSH), Sialidase (classification), Sialidase (génétique), Sialidase (isolement et purification), Sialidase (métabolisme), Sous-type H1N1 du virus de la grippe A (enzymologie), Sous-type H3N2 du virus de la grippe A (enzymologie), Test ELISA (MeSH), Virus de la grippe A (classification), Virus de la grippe A (enzymologie), Virus de la grippe A (immunologie).
- MESH :
- enzymologie : Sous-type H1N1 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Virus de la grippe A.
- génétique : Sialidase.
- immunologie : Virus de la grippe A.
- isolement et purification : Sialidase.
- métabolisme : Sialidase.
- virologie : Grippe humaine.
- épidémiologie : Grippe humaine.
- classification : Humains, Sialidase, Test ELISA, Virus de la grippe A.
English descriptors
- KwdEn :
- Enzyme-Linked Immunosorbent Assay (MeSH), Humans (MeSH), Influenza A Virus, H1N1 Subtype (enzymology), Influenza A Virus, H3N2 Subtype (enzymology), Influenza A virus (classification), Influenza A virus (enzymology), Influenza A virus (immunology), Influenza, Human (epidemiology), Influenza, Human (virology), Neuraminidase (classification), Neuraminidase (genetics), Neuraminidase (isolation & purification), Neuraminidase (metabolism).
- MESH :
- chemical , classification : Neuraminidase.
- classification : Influenza A virus.
- enzymology : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A virus.
- epidemiology : Influenza, Human.
- chemical , genetics : Neuraminidase.
- immunology : Influenza A virus.
- chemical , isolation & purification : Neuraminidase.
- chemical , metabolism : Neuraminidase.
- virology : Influenza, Human.
- Enzyme-Linked Immunosorbent Assay, Humans.
Abstract
An ELISA assay was developed to allow the rapid and accurate identification of human influenza A N1 and N2 neuraminidases. Initial testing using a fetuin pre-coating of wells correctly identified 81.7% of the neuraminidase type from a series of human A(H1N1), A(H1N2) and A(H3N2) viruses. This result could be improved to detect the neuraminidase subtype of almost all human influenza A viruses from a large panel of viruses isolated from 2000 to 2005, if the fetuin pre-coating was removed and the viruses were coated directly onto wells. This method is simple, rapid and can be used to screen large numbers of currently circulating human influenza A viruses for their neurraminidase subtype and is a good alternative to RT-PCR.
DOI: 10.1016/j.vaccine.2006.05.047
PubMed: 16860447
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA.</title><author><name sortKey="Barr, I G" sort="Barr, I G" uniqKey="Barr I" first="I G" last="Barr">I G Barr</name><affiliation wicri:level="1"><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Victoria 3052, Australia. Ian.Barr@influenzacentre.org</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Victoria 3052</wicri:regionArea><wicri:noRegion>Victoria 3052</wicri:noRegion></affiliation></author><author><name sortKey="Mccaig, M" sort="Mccaig, M" uniqKey="Mccaig M" first="M" last="Mccaig">M. Mccaig</name></author><author><name sortKey="Durrant, C" sort="Durrant, C" uniqKey="Durrant C" first="C" last="Durrant">C. Durrant</name></author><author><name sortKey="Shaw, R" sort="Shaw, R" uniqKey="Shaw R" first="R" last="Shaw">R. Shaw</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16860447</idno><idno type="pmid">16860447</idno><idno type="doi">10.1016/j.vaccine.2006.05.047</idno><idno type="wicri:Area/Main/Corpus">003D65</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003D65</idno><idno type="wicri:Area/Main/Curation">003D65</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003D65</idno><idno type="wicri:Area/Main/Exploration">003D65</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA.</title><author><name sortKey="Barr, I G" sort="Barr, I G" uniqKey="Barr I" first="I G" last="Barr">I G Barr</name><affiliation wicri:level="1"><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Victoria 3052, Australia. Ian.Barr@influenzacentre.org</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Victoria 3052</wicri:regionArea><wicri:noRegion>Victoria 3052</wicri:noRegion></affiliation></author><author><name sortKey="Mccaig, M" sort="Mccaig, M" uniqKey="Mccaig M" first="M" last="Mccaig">M. Mccaig</name></author><author><name sortKey="Durrant, C" sort="Durrant, C" uniqKey="Durrant C" first="C" last="Durrant">C. Durrant</name></author><author><name sortKey="Shaw, R" sort="Shaw, R" uniqKey="Shaw R" first="R" last="Shaw">R. Shaw</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay (MeSH)</term><term>Humans (MeSH)</term><term>Influenza A Virus, H1N1 Subtype (enzymology)</term><term>Influenza A Virus, H3N2 Subtype (enzymology)</term><term>Influenza A virus (classification)</term><term>Influenza A virus (enzymology)</term><term>Influenza A virus (immunology)</term><term>Influenza, Human (epidemiology)</term><term>Influenza, Human (virology)</term><term>Neuraminidase (classification)</term><term>Neuraminidase (genetics)</term><term>Neuraminidase (isolation & purification)</term><term>Neuraminidase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Grippe humaine (virologie)</term><term>Grippe humaine (épidémiologie)</term><term>Humains (MeSH)</term><term>Sialidase (classification)</term><term>Sialidase (génétique)</term><term>Sialidase (isolement et purification)</term><term>Sialidase (métabolisme)</term><term>Sous-type H1N1 du virus de la grippe A (enzymologie)</term><term>Sous-type H3N2 du virus de la grippe A (enzymologie)</term><term>Test ELISA (MeSH)</term><term>Virus de la grippe A (classification)</term><term>Virus de la grippe A (enzymologie)</term><term>Virus de la grippe A (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Neuraminidase</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Neuraminidase</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sialidase</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Neuraminidase</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sialidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Neuraminidase</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Sialidase</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Humains</term><term>Sialidase</term><term>Test ELISA</term><term>Virus de la grippe A</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An ELISA assay was developed to allow the rapid and accurate identification of human influenza A N1 and N2 neuraminidases. Initial testing using a fetuin pre-coating of wells correctly identified 81.7% of the neuraminidase type from a series of human A(H1N1), A(H1N2) and A(H3N2) viruses. This result could be improved to detect the neuraminidase subtype of almost all human influenza A viruses from a large panel of viruses isolated from 2000 to 2005, if the fetuin pre-coating was removed and the viruses were coated directly onto wells. This method is simple, rapid and can be used to screen large numbers of currently circulating human influenza A viruses for their neurraminidase subtype and is a good alternative to RT-PCR.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16860447</PMID><DateCompleted><Year>2007</Year><Month>01</Month><Day>23</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0264-410X</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>44-46</Issue><PubDate><Year>2006</Year><Month>Nov</Month><Day>10</Day></PubDate></JournalIssue><Title>Vaccine</Title><ISOAbbreviation>Vaccine</ISOAbbreviation></Journal><ArticleTitle>The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA.</ArticleTitle><Pagination><MedlinePgn>6675-8</MedlinePgn></Pagination><Abstract><AbstractText>An ELISA assay was developed to allow the rapid and accurate identification of human influenza A N1 and N2 neuraminidases. Initial testing using a fetuin pre-coating of wells correctly identified 81.7% of the neuraminidase type from a series of human A(H1N1), A(H1N2) and A(H3N2) viruses. This result could be improved to detect the neuraminidase subtype of almost all human influenza A viruses from a large panel of viruses isolated from 2000 to 2005, if the fetuin pre-coating was removed and the viruses were coated directly onto wells. This method is simple, rapid and can be used to screen large numbers of currently circulating human influenza A viruses for their neurraminidase subtype and is a good alternative to RT-PCR.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Barr</LastName><ForeName>I G</ForeName><Initials>IG</Initials><AffiliationInfo><Affiliation>WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Victoria 3052, Australia. Ian.Barr@influenzacentre.org</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McCaig</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Durrant</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Shaw</LastName><ForeName>R</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>06</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Vaccine</MedlineTA><NlmUniqueID>8406899</NlmUniqueID><ISSNLinking>0264-410X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber><NameOfSubstance UI="D009439">Neuraminidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053122" MajorTopicYN="N">Influenza A Virus, H3N2 Subtype</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009439" MajorTopicYN="N">Neuraminidase</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>7</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>1</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>7</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16860447</ArticleId><ArticleId IdType="pii">S0264-410X(06)00642-6</ArticleId><ArticleId IdType="doi">10.1016/j.vaccine.2006.05.047</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li></country></list><tree><noCountry><name sortKey="Durrant, C" sort="Durrant, C" uniqKey="Durrant C" first="C" last="Durrant">C. Durrant</name><name sortKey="Mccaig, M" sort="Mccaig, M" uniqKey="Mccaig M" first="M" last="Mccaig">M. Mccaig</name><name sortKey="Shaw, R" sort="Shaw, R" uniqKey="Shaw R" first="R" last="Shaw">R. Shaw</name></noCountry><country name="Australie"><noRegion><name sortKey="Barr, I G" sort="Barr, I G" uniqKey="Barr I" first="I G" last="Barr">I G Barr</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003D02 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003D02 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:16860447
|texte= The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16860447" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |